ESAT-6 Drives MMP-10 Gene Expression and Secretion in Tuberculosis

MMP - 10 gene expression and secretion in tuberculosis

Tuberculosis (TB) causes disease worldwide and multi-drug resistance is an increasing problem. Matrix metalloproteinases (MMPs), particularly the collagenase MMP-1, cause lung extracellular matrix destruction which drives disease transmission and morbidity. The role in such tissue damage of the stromelysin MMP10, a key activator of the collagenase MMP-1, was investigated in direct Mycobacterium...

Cloning and Expression of Mycobacterium Tuberculosis ESAT-6 in Prokaryotic System

  The identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. This study was designed for cloning and expression of ESAT-6 as a potent antigen of Mycobacterium tuberculosis. Selected gene (Rv3875) was amplified by PCR and product was ligated into expressing plasmid vector pQE30 and recombinant pQE30-ES plasmi...

cloning, expression and purification of mycobacterium tuberculosis esat-6 and cfp-10 antigens.

background and objectives: esat-6 (6-kdaearly secretory antigenic target) and cfp-10 (10-kda culture filtrate protein) have been described as dominant antigens recognized by t-cells and considered as virulence factors in mycobacterium tuberculosis . the aim of this study was to clone, express and purify recombinant esat-6 andcfp-10 proteins of m. tuberculosis in soluble form. materials and meth...

Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins

Background: Macrophage immune responses are affected by the secretory proteins of Mycobacterium tuberculosis (Mtb). This study aimed to examine the immune responses of macrophages to Mtb secretory antigens, namely ESAT-6, CFP-10, and ESAT-6/CFP-10.Methods: THP-1 cells (a human monocytic cell line) were cultured and differentiated to macrophages by phorbol 12-myristate 13-acetate. The cytotoxici...

expression and purification of recombinant mycobacterium tuberculosis (tb) antigens, esat-6, cfp-10 and esat- 6/cfp-10 and their diagnosis potential for detection of tb patients